HPLC -MS/MS for the determination of atorvastatin and its metabolites concentrations in human plasma
10.13699/j.cnki.1001-6821.2015.12.031
- VernacularTitle:高效液相色谱质谱联用法测定人血浆中阿托伐他汀及代谢产物的质量浓度
- Author:
Xue-Yi CHEN
1
;
Guang-Tao HAO
;
Long-Mei CHENG
;
Ying-Fu ZHANG
;
Ya-Fei XU
;
Ze-Yuan LIU
;
Heng-Yan QU
Author Information
1. 军事医学科学院 附属医院 临床药理学研究室
- Keywords:
HPLC - MS/MS;
atorvastatin;
metabolite;
ortho-hydroxyatorvastatin;
para-hydroxyatorvastatin
- From:
The Chinese Journal of Clinical Pharmacology
2015;(12):1184-1187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the mass concentration of atorvasta-tin and its metabolites in human plasma by HPLC -MS/MS method. Methods The solid-phase extraction was adopted to process the blood sample, and the XTerra ? MS C18 column was used, with 95%acetonitrile-5 mmol? L-1 ammonium bicarbonate as the mobile phase, gradient elution with positive ion scan and multiple reaction monitoring mode mass concentration of the sample was measured.The specificity, standard curve and lower limit of quantification, accuracy and recovery rate, matrix effect and stability were investigated.Results The standard curve of atorvastatin and its metabolites were Y =1.82 X-2.94 ×10 -3 (r=0.999 5),Y=0.28X -4.14 ×10 -5 (r =0.999 7),Y=0.60X +1.11 ×10 -3 ( r=0.997 4 ) , and they showed a good linearship between 0.1 and 40.0 ng? mL-1 .The lowest concentration was 0.1 ng? mL-1 . The intra and inter-day RSD were less than 15%, the average recovery was higher than 80%, the stability was good.Conclusion The method is simple, rapid, sensitive and accurate and specific, suitable for phar-macokinetics study of atorvastatin and metabolites dynamics in vivo .
