Growth inhibition of gastric cancer cell lines SNU-1 induced by molecular targeted drug combined with NF-kβinhibitor
10.13699/j.cnki.1001-6821.2015.12.027
- VernacularTitle:分子靶向药物联合核转录因子 kβ抑制剂对胃癌细胞系 SNU－1的抑制作用
- Author:
Xiao-Meng CHEN
1
;
Meng ZHANG
;
Peng-Li FAN
;
Yu-Hua QIN
;
Hong-Wei ZHAO
Author Information
1. 河南省人民医院药学部
- Keywords:
molecular targeted drug;
sorafenib;
lapatinib;
ammonium pyrrolidinedithiocarbamate
- From:
The Chinese Journal of Clinical Pharmacology
2015;(12):1169-1172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence on proliferation, apoptosis and cell cycle of gastric cancer cell lines SNU-1 induced by molecular targeted drug sorafenib and lapatinib combined with ammonium pyrrolidinedithiocarbamate ( PDTC).Methods Groups were divided as follows:blank group ( A group ) , fore test groups, include B group, sorafenib+lapatinib(3,3μmol? L-1, respectively),C group, sorafenib+PDTC(3,50 μmol? L-1,respectively),D group, lapatinib +PDTC(3, 50 μmol? L-1 , respectively ) , and E group, sorafenib +lapatinib +PDTC(3,3,50 μmol? L-1 ,respectively) and three different concentra-tions of sorafenib and lapatinib ( 1.5 , 6.0 , 12.0 μmol? L-) , PDTC (25,100,200 μmol? L-1 ) as control groups, respectively.The inhibi-tion of gastric cell line SNU-1 was detected by MTT.The cell cycle and apoptosis were disclosed by flow cytometry, respectively.Results There were some certain inhibition of gastric cancer cell line SNU-1 induced by different groups.The differences had significantly in three drugs combi-nation groups on the rate of cell inhibition, the S-phase cells decreased, and increased apoptosis ( all P <0.05 ) . Conclusion Combining with three drugs can effectively inhibit the proliferation and induce apoptosis of gastric cancer cell SNU-1.
