Anti-inflammatory effect of Danshiliuhao decoction on pigment stones of Guinea pigs
10.13699/j.cnki.1001-6821.2015.12.025
- VernacularTitle:胆石六号汤剂对豚鼠胆色素结石模型胆囊的抗炎作用
- Author:
Long-Zhong LIU
1
;
Ying QIAN
;
Shan-Shan HU
;
Ze-Hui CHEN
;
Ying-Biao TIAN
Author Information
1. 遵义医学院 附属医院 药剂科
- Keywords:
pigment gallstone;
Danshiliuhao decoction;
tumor necrosis factor-α;
nuclear factor-κB
- From:
The Chinese Journal of Clinical Pharmacology
2015;(12):1162-1164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Danshiliuhao decoction on the expression of tumor necrosis factor -α( TNF -α) and nuclear factor-κB( NF -κB ) in the bile pigment with stone in Guinea pigs. Methods The female Guinea pigs were randomly divided into three groups.The blank group ( n =10 ) , the model group ( n =15 ) and test group ( Danshiliuhao,n=15 ) .The latter two groups of the Guinea pigs were both modeled as the bile pigment stones model by feeding them with a different diet .The Guinea pigs in test group were given Danshi-liuhao decoction 7.7 g? kg-1? d-1 for 30 days.The rate of stone formation and the expression of the gallbladder TNF-αand NF-κB were observed af-ter administration by immunohistochemistry.Results The rate of stone formation was 90.62% in model group, significantly higher than that (26.76%) in the blank group.The results suggested that the model for Danshiliuhao is successfully built and the drug is an effective agent for anti-gallstones.The expression of the TNF -αand NF -κB in test group were significantly lower than model group. The difference was statistically significant ( P<0.05 ) .Conclusion Danshiliuhao Decoc-tion significantly reduced the rate of pigment stone formation in Guinea pigs.This may be related to the decreased expression of TNF-αand NF-κB proteins in gallbladders of Guinea pigs by the drug.
