Effects of toll like receptor 4 interference on lysophosphatidic acid -stimulated phenotypic modulation of vascular smooth muscle cells
10.13699/j.cnki.1001-6821.2015.12.022
- VernacularTitle:Toll样受体4基因干预对于溶血磷脂酸诱导的大鼠平滑肌细胞表型转化的影响
- Author:
Cheng-Fang HUANG
1
;
Bo YANG
;
Dan JIANG
;
Zhi-Bin ZHOU
Author Information
1. 武汉科技大学 附属天佑医院 神经内科
- Keywords:
toll like receptor;
RNA interference;
lysophosphatidic acid;
vascular smooth muscle cell;
phenotypic modulation
- From:
The Chinese Journal of Clinical Pharmacology
2015;(12):1150-1153,1161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of special siRNA target-ting toll like receptor 4 ( TLR4) gene( TLR4-siRNA) on LPA-stimu-lated phenotypic modulation of vascular smooth muscle cells.Methods depressed by TLR4 -siRNA transfection, which held back the down -regulation of SMA -αgene expression, de-pressed up-regulation of osteopontin gene expression induced by lysophosphatidic acid, but there were no cell shape changes as compared with dedifferentiated RASMCs.Conclusion LPA-stimulated phenotypic modulation of VSMC may partly involve TLR4 pathway.Inhibition of inflammatory and immunity reaction might be a potential novel target for therapeutic intervention for arteriosclerosis.
