Effect of erythropoietin on Fas/FasL expression in brain tissues of neonatal rats with hypoxic ischemic brain damage
10.13699/j.cnki.1001-6821.2015.12.021
- VernacularTitle:促红细胞生成素对缺氧缺血性脑损伤新生大鼠脑组织 Fas／FasL表达的影响
- Author:
Jing ZHANG
1
;
Rui HUANG
;
Ling HAO
;
Na LIU
;
Shao-Juan DAI
Author Information
1. 河北医科大学 第一医院 儿科
- Keywords:
erythropoietin;
hypoxic ischemic brain damage;
factor-related apoptosis;
factor-related apoptosis ligand
- From:
The Chinese Journal of Clinical Pharmacology
2015;(12):1146-1149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of erythropoietin ( EPO) on factor -related apoptosis and its ligand ( Fas/FasL ) expression in brain tissues of neonatal rats with hypoxic ischemic brain damage ( HIBD ) .Methods One hundred and twenty newborn rats ( 7 days years old ) were selected and randomly divided into 3 groups: sham group, hypoxic ischemic brain damage group ( model group ) and EPO treatment group ( test group ) , each group was further divided into five time points:6, 12, 24, 48 and 72 h after HIBD model was established. Eight rats in each group were killed at different time points.After which samples of brain tissues were harvested.Change of brain cell morphology was observed by HE staining and expression of Fas/FasL was observed by immunohistochemical staining.Results In model group, expressions of Fas and FasL reached the peak at 48 h after HIBD model was established and decreased at 72 h.Compared with sham group, the expressions of Fas and FasL in model group at each time point were much higher, and the difference was statistically significant ( P <0.05 ) .In test group, expressions of Fas and FasL at each time point were significantly lower than in model group ( P <0.05 ) .Conclusion Cell apoptosis in the brain tissue after hypoxic -ischemic damage could be remarkably inhi-bited by EPO through reduction of Fas and FasL expression in brain tissue of neonatal rats with HIBD.
