Expression analysis of a disintegrin and metalloprotease 9 during in the mice induced by acetaminophen
10.13699/j.cnki.1001-6821.2015.12.020
- VernacularTitle:解整合素－金属蛋白酶9在对乙酰氨基酚诱导的小鼠急性肝损伤中的表达分析
- Author:
San-Qiang LI
1
;
Hong-Mei HAN
;
Hua-Jie LU
;
Shou-Min XI
Author Information
1. 河南科技大学 医学院 肝脏损伤与修复分子医学重点实验室
- Keywords:
acetaminophen;
a disintegrin and metalloproteases 9;
acute liver injury;
expression analysis
- From:
The Chinese Journal of Clinical Pharmacology
2015;(12):1143-1145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expressed dynamic change and roles of a disintegrin and metalloprotease 9 ( ADAM9) during acute liver injury induced by acetaminophen.Methods Fifty mice were randomly divided into two groups:normal group (n=10) and experiment group (n=40). The mice in experiment group were respectively drawn blood by removing the eyeballs and serum was separated to detect the activity of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) at 6, 24, 42 and 72 h after intraperitoneal injection of acetaminophen 550 mg? kg-1 .The activity of serum AST and ALT in the mice of normal group were also detected.The expression of hepatic ADAM9 at protein and mRNA levels were detected by western blot and RT -PCR method in the mice of normal group and experiment group at different time points after acetaminophen injection.Results The expression of ADAM9 protein and mRNA were significantly down -regulated at 6 h ( P<0.05) , and the expression of ADAM9 protein and mRNA reached the lowest level at 24 h ( P <0.05 ) . After that, the expression of ADAM9 protein and mRNA were gradually increased.The expression of ADAM9 was significantly increased at 42 h (P<0.05),and then recovered closed to the normal level at 72 h after acetaminophen injection.Conclusion ADAM9 is remarkably differently ex-pressed during acute liver injury induced by acetaminophen, which indicates that ADAM9 may play an important pro-tective role during acute liver injury induce by acetaminophen in mice.
