Experimental study of the improvement on neurologic function of curcumin preconditioning on acute spinal cord injury in mice
10.13699/j.cnki.1001-6821.2015.11.015
- VernacularTitle:姜黄素预处理保护急性脊髓损伤小鼠神经功能的实验研究
- Author:
Nan-Wen ZHANG
1
;
Jian YE
;
Yan-Liang CAI
;
Yun-Jiao HE
;
Jin-Tian WANG
;
Jian-Qing CHEN
;
Zeng-Kai WU
;
Gui-Cai WEI
Author Information
1. 福建医科大学
- Keywords:
acute spinal cord injury;
curcumin;
oxidative stress;
infla-mmation
- From:
The Chinese Journal of Clinical Pharmacology
2015;(11):950-953
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of curcumin on neuronal protective function in acute spinal cord model ( ASCI) mice. Methods Divide healthy and clean class mice into sham -operated group,model group, control group ( methylprednisolone, 40 mg · kg-1 ) and treatment group ( curcumin 200 mg · kg-1 ).Each mice respectively received injection in intraperitoneal with drugs at 30 min before the sur-gery.Then, experimental animal models was established following im-proved Allen′s model.The behavior of the model mice was performed.The nuclear factor E2-related faetor 2 ( Nrf 2 ) antibody expression was examined with Western blot.And expression of tumor necrosis factor-α( TNF-α) and interleukin-6 ( IL-6 ) were examined with enzyme-linked immunosorbent assay.Results Compared to model group, beha-vior score,neurologic function,the expression of Nrf 2 and TNF -αin treatment group achieved significant improvement.The IL-6 expression in treatment group only revealed weaker in 8 h, but no notable difference compared to model group after 8 h.Conclusion The preliminary study shows that the way of curcumin preconditioning to improve neurologic function may by decreasing inflammatory factor and raising the expression of Nrf2.
