Study on the inhibitory effect and potential mechanism of Tetramethylpyrazin in combination with cisplatin on the tumor growth and angiogenesis in mouse Lewis lung cancer
10.13699/j.cnki.1001-6821.2015.11.014
- VernacularTitle:川芎嗪联合顺铂抗肿瘤血管生成的作用机制研究
- Author:
Bu WANG
1
;
Zhi-Lin ZHANG
;
Zhi-Hua ZHANG
;
Xiu-Long ZHANG
;
Jian-Hua TANG
;
Kai-Yang LIU
Author Information
1. 河北北方学院
- Keywords:
lung carcinoma;
vascular endothelial growth factor;
Tetramethylpyrazin;
a disintegrin and metalloprotei-nase with thrombospondin typeⅠmotif
- From:
The Chinese Journal of Clinical Pharmacology
2015;(11):946-949
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Tetramethylpyrazin ( TMP) combined with cisplatin( DDP) on expression of a disintegrin and metalloproteinase with thrombospondin typeⅠmotif ( ADAMTS1 ) and vascular endothelial growth factor ( VEGF ) of Lewis lung cancer rats.Methods The Lewis lung carcinoma model of c57 bl/6 were produced by intraperitoneal injection of Lewis lung cancer cells in right axillary, per 0.2 mL.Then the 56 tumor-bearing mice were randomized into 4 groups: model group ( 0.9%NaCl, 0.2 mL ) , TMP group ( 100 mg· kg-1, 0.2 mL),DDP group(2 mg· kg -1, 0.2 mL),TMP+DDP group( doses as above, n=14).Different administration were served from day 5 transplantation and all mice were sacrificed after 19 days.The weight and volume of transplanted tumors were recorded.And the expressions of VEGF and ADAMTS1 were detected by immunhistochem-isty.Results Compared with model group, the growth of transplanted tumors in TMP group, DDP group, TMP+DDP group were significantly inhibited.The weight of tumor body in the three groups were markedly lower than that of model group.The inhibitory effect of TMP combined with DDP on tumor growth was enhanced com-pared with TMP or DDP alone treatment.The expression levels of VEGF were significantly lower but ADAMTS1 was sig-nificantly higher in TMP group,DDP group,TMP+DDP group than that in model group ( P<0.05 ).Pearson correla-ted analysis showed that the expression VEGE and ADAMTS1 negatively correlated(P<0.05).Conclusion TMP has synergetic inhibitory effect with DDP on transplanted tumor growth of Lewis lung carcinoma in mice.The action mecha-nism may be due to down-regulate the VEGF expression and up-regulate the ADAMTS1 expression,inhibiting tumor angiogenesis.
