Inhibitory effect of Tetramethylpyrazine in combination with cisplatin on growth of Lewis lung adenocarcinoma in mice
10.13699/j.cnki.1001-6821.2015.11.013
- VernacularTitle:川芎嗪联合顺铂对 Lewis肺腺癌小鼠抑制生长的作用
- Author:
Chang-Hong ZHANG
1
;
Zhi-Lin ZHANG
;
Zhi-Hua ZHANG
;
Xiu-Long ZHANG
;
Jian-Hua TANG
;
Kai-Yang LIU
Author Information
1. 河北北方学院
- Keywords:
Tetramethylpyrazine;
lung cancer;
hypoxia -inducible factor -1α;
basic fibroblast growth factor;
vasohibin-1
- From:
The Chinese Journal of Clinical Pharmacology
2015;(11):942-945
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Tetramethylpyrazine combined with cisplatin on Lewis lung adenocarcinoma by the change of hypoxia-inducible factor 1 alpha ( HIF -1α) , basic fibroblast growth factorb ( b-FGF) ,vasohibin-1 ( VASH-1 ) in mice tumor tissues and explore the mechanism of anti -tumor.Methods Forty -eight mice were randomly divided into 4 groups, control group, cisplatin group, Tetramethylpyrazine group and combined group, 12 rats in each group, cisplatin at a dose of 2 mg· kg -1 , Tetramethylpyrazine at a dose of 100 mg· kg-1 , combination group( the doses as above).All mice were sac-rificed after treatment for two weeks and these tumors were obtained.The weighed, and the tumor inhibitory was calculated.The expressions of HIF-1α, b -FGF and VASH -1 were determined by immunohisto-chemistry after two weeks administration.Results Compared with the control group, the tumor inhibition rate in experimental groups was signi-ficantly increased ( P<0.05 ) , and was highest in combination group.The expression of HIF -1α, b -FGF and VASH -1 in the cisplatin group, Tetramethylpyrazine group and combined group were decreased obviously compared with control group( P<0.05).And the levels of the expression were the lowest in the combined group and the highest in control group.HIF -1αis positively correlated with b -FGF ( P <0.05 ) , b -FGF is positively correlated with VASH-1 ( P <0.05 ) , HIF-1αis uncorrelated with VASH-1.Conclusion Tetramethylpyrazine combined with cisplatin decreased the expression of HIF-1α, thereby reducing the expression of b-FGF and VASH-1 , inhibiting the tumor growth synergistically.
