Study on the mechanism of Hep -2 cells proliferation inhibition induced by indoel-3-carbinol
10.13699/j.cnki.1001-6821.2015.11.010
- VernacularTitle:吲哚－3－甲醇对人喉癌 Hep－2细胞增殖的抑制作用研究
- Author:
Xue-Jun LIU
1
;
Sai-Yu HUANG
;
Jin-Jian GAO
;
Qi-Jun FAN
;
Li-Yan NI
;
Bo-Bei CHEN
Author Information
1. 温州医科大学 附属第二医院 育英儿童医院 耳鼻咽喉科
- Keywords:
indole -3 -carbinol;
Hep -2 cell;
cell proliferation;
cell apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2015;(11):932-934,959
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of indoel -3 -carbinol on Hep-2 cells in vitro.Methods There was divided into 5 groups, the 4 test groups (the concerntration of indoel-3-carbinol, 0, 50, 100, 150μmol· L-1) and the control group(dimethyl sulfoxide).The inhibition of cell proliferation was assayed by method of WST-1.And the apopto-sis was shown with Hochest33258 staining and flow cytometry.The mechanism of apoptosis, and the expressions of the livin protein were detected by Western blotting assay.Results After being disposed by the test groups with 4 different concerntration of indoel-3-carbinol and the control group, the apoptosis rate of Hep-2 cells were(3.95 ±0.68)%, ( 8.54 ±1.35 )%, ( 21.28 ±2.32 )%, ( 38.65 ±4.85 )%, and (4.26 ±0.58)%, respectively.The indoel -3 -carbinol could inhibit the proliferation of Hep-2 cells with the dose-dependent manner and the apoptosis could be induced too.Furthermore, the expressions of Livin appeared to be decreased significantly as compared with the control group.Conclusion The indoel-3-carbinol could induce apoptosis of Hep-2 cells in the manner of dose-dependent, and Livin might play a role during above processes.
