Study on the combining force of interaction between cefotaxime and bovine serum albumin
10.13699/j.cnki.1001-6821.2015.11.007
- VernacularTitle:注射用头孢噻肟钠与牛血清白蛋白相互作用研究
- Author:
Su-Feng ZHANG
1
;
Ming LI
;
Huai-Bin ZHANG
Author Information
1. 青州市人民医院 药剂科
- Keywords:
cefotaxime sodium;
bovine serum albumin;
spectral analysis;
combining force
- From:
The Chinese Journal of Clinical Pharmacology
2015;(11):921-923
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the interaction between cefotaxime sodium for injection(CTX) and bovine serum albumin(BSA) in tris-HCl buffer of pH 7.40.Methods The fluorescence intensity of the interac-tion between BSA and CTX was studied by the fluorescence spectrometry.The fluorescence intensity were analyzed in according to Stern-Volmer equation, double logarithmic equation and thermodynamic equation.Results The fluorescence of BSA was quenched by CTX.At the 299 and 307 K, the quenching rate constant ( Kq ) was 3.07 ×1012 , 3.48 ×1012 L· mol-1 · s-1 ,and the binding constant(KA ) was 5.75 ×103 , 4.47 × 103 L · mol-1 , respectively.The value of thermodynamic parameters (△G,△H and△S) were less than zero.Conclusion The CTX and BSA formed the ground state complex, and the combining force between CTX and BSA was Van der Waals force and hydrogen bond.
