Molecular epidemiology and resistance of oxacillin susceptible methicillin-resistant Staphylococcus aureus isolates
10.13699/j.cnki.1001-6821.2015.11.005
- VernacularTitle:苯唑西林敏感甲氧西林耐药金黄色葡萄球菌的分子流行病学及耐药性分析
- Author:
Lan-Qing CUI
1
;
Yun LI
;
Feng XUE
;
Jia ZHANG
;
Yuan LV
Author Information
1. 北京大学 第一医院 临床药理研究所
- Keywords:
Staphylococcus aureus;
methicillin-resistant;
oxacillin;
resistance;
molecular typing
- From:
The Chinese Journal of Clinical Pharmacology
2015;(11):912-915
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the resistance, molecular charac-teristics of oxacillin susceptible methicillin-resistant Staphylococcus au-reus ( OS-MRSA) strains isolated 2011-2012 in China.Methods A total of 297 methicillin -resistant Staphylococcus aureus ( MRSA ) were collected in the Ministry of Health National Antimicrobial Resistance Sur-veillance Net program and the susceptibility test were performed by agar dilution method.All the oxacillin-susceptible MRSA strains were con-firmed to be Staphylococcus aureus by detecting nuc gene.The molecular typing methods included SCCmec and spa typing.Results A total of 14 isolates ( 4.9%) were oxacillin susceptible methicillin-resistant Staphy-lococcus aureus among 288 MRSA isolates and most of them were from se-cretion (50%, 7/14) and blood (28.6%, 4/14).The susceptibility rate to gentamicin, amikacin, rifampicin and trimethroprim/sulfamethox-azole was more than 90% and the rate to quinolones, tetracycline and chloramphenicol was about 80%, 28.6%, 28.6%, respectively.Moreo-ver, the resistance rate to erythromycin, azithromycin and clindamycin was more than 90%.PCR revealed that 14 isolates were Staphylococcus aureus and mecA positive and the most predominant clone was SCCmecⅤ-spat437 (42.9%,6/14).Conclusion The most predominant clone of the OS-MRSA isolates in China was SCCmecⅤ-spat437; most of OS-MRSA isolates were susceptible to the majority of antibacterial agents except macrolides, tetracycline and chloramphenicol.
