Protective effect of Tongxinluo capsule on diabetic peripheral neuropathy in mice
10.13699/j.cnki.1001-6821.2015.10.032
- VernacularTitle:通心络胶囊改善小鼠糖尿病周围神经病变的保护作用
- Author:
Chao WANG
1
;
Hui-Xin ZHANG
;
Han-Ying XING
;
Xing WANG
;
Min LIU
Author Information
1. 河北省人民医院 老年医学重点实验室
- Keywords:
Tongxinluo capsule;
diabetic peripheral neuropathy;
inflammatory cytokines;
nuclear factor kappa B pathway
- From:
The Chinese Journal of Clinical Pharmacology
2015;(10):874-877
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Tongxinluo capsule on diabetic peripheral neuropathy in mice .Methods KK/Upj -Ay mice were randomly divided into 4 groups,model, Tongxinluo low, middle, high doses groups (0.8, 0.4, 0.2 g? kg -1 ).C57BL/6 mice were se-lected as control group.Mice were given intragastrically for 12 weeks. Motor nerve conduction velocity ( MNCV ) and sensory nerve conduction velocity ( SNCV) were detected.The expression of tumor necrosis factor alpha ( TNF -α) , interleukin 6 ( IL -6 ) and interleukin 1 beta (IL-1β) and nuclear factor kappa B (NF-κB) in sciatic nerve were examined by real time PCR and Western blot .Results Compared with model group , MNCV and SNCV were faster ( P<0.05 , P<0.01 ) .The TNF-α, IL-6 , IL-1βand NF-κB expression significantly were de-creased in Tongxinluo group ( P < 0.05 , P < 0.01 ) . Conclusion Tongxinluo capsule inhibits inflammatory cytokines expres-sion in diabetic peripheral neuropathy mice by inhibiting NF -κB pathway , to ameliorates diabetic peripheral neuropathy .
