Protective effect of ω-3 polyunsaturated fatty acids on neonatal rats with hypoxic-ischemic brain injury
10.13699/j.cnki.1001-6821.2015.10.030
- VernacularTitle:ω-3多不饱和脂肪酸对新生鼠缺血缺氧性脑损伤的保护作用研究
- Author:
Jing ZHANG
1
;
Zhen-Long ZHU
;
Chang-Jun REN
;
Shao-Juan DAI
Author Information
1. 河北医科大学 第一医院 儿科
- Keywords:
ω-3 polyunsaturated fatty acids;
hypoxia -ischemia brain damage;
inflammatory reaction;
neurobehavioral function
- From:
The Chinese Journal of Clinical Pharmacology
2015;(10):865-868
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of ω-3 polyunsaturated fatty acid (ω-3 PUFAs ) on hypoxic ischemic brain damage(HIBD) in neonatal rats.Methods Eighteen pregnant rats were randomly divided into 3 groups, control group (6 pregnant rats, 26 new-born rats), sham operation group (6 pregnant rats, 24 neonatal rats), intervention group ( 6 pregnant rats , 28 newborn rats ) . Intervention group was administration with ω-3PUFAs (1 mg? g-1? d -1 ) by ga-vage , from 2 d after pregnancy to childbirth after 14 d.The rats in con-trol group and sham operation group were given the same amount of 0.9%NaCl.The newborn mice of 7 d old were made into HIBD model . Brain edema was measured using the gravimetric method .The expression of inducible nitric oxide synthase ( iNOS) and interleukin -6 ( iL-6 ) in brain tissue were detected by Western blot .The learning and memory function was assessed by the modified Morris water maze .Results Com-pared with control group , the newborn mice brain edema degree of inter-vention group was more ease , the difference was statistically significant (P<0.05).The expression of iNOS and interleukin -6 in newborn rat cerebral cortex of intervention group was more higher , the difference was statistically significant ( P <0.05 ) .The rats of learning and memory abilityininterventiongroupwasstronger,thedifferencewasstatisticallysignificant(P<0.05).Conclusion ω-3 polyunsaturated fatty acids may inhibit the expression of interleukin -6 and iNOS, and play a protective role in HIBD of newborn rat.
