Effects of histone deacetylase inhibitor on the expression of Notch1 receptor and the ligands of jagged1 and jagged 2
10.13699/j.cnki.1001-6821.2015.10.029
- VernacularTitle:组蛋白脱乙酰化酶抑制剂对 Notch1受体及jagged1与 jagged2配体表达的影响
- Author:
Rui-Juan ZHAO
1
;
Lian QIAO
;
Yue YANG
;
Lei ZHAO
;
Li-Hong WANG
;
Li-Na YAN
;
Zhi-Hua ZHANG
;
Chang-Lai HAO
Author Information
1. 承德医学院 附属医院 血液病科
- Keywords:
multiple myeloma;
valproic acid sodium;
Notch1 receptor jagged1 jagged2 RPMI8226 cell
- From:
The Chinese Journal of Clinical Pharmacology
2015;(10):861-864
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of valproic acid ( VPA ) on the expression of Notch receptor and ligand in multiple myeloma ( MM ) cell line RPMI8226.Methods RPMI8226 cell line was cultured , four groups have been set up in the experiment according to the concentrations (0, 2, 4, 8 mmol? L -1 ) of VPA.When RPMI8226 cell exposed to different concentrations of VPA for 24, 48 and 72 h, the cell proliferation inhibition was measured with 3-(4,5-dimethylthiazol -2-yl)-2,5-diphemyltetra-zolium Bromide ( MTT) assay method.The change of the expression of mRNA and protein level of Notch 1 , jagged1 and jagged2 were detected by RT-PCR and Western Blot .Results The proliferation inhibition of VPA on RPMI 8226 cell in a concentration -and time-dependent manner.Compared with control group(blank), incuba-ted with different concentrations (2, 4, 8 mmol? L-1 ) of VPA for 48 h, the expression of Notch1,jagged1,jagged2 mRNA and protein were gra-dually decreased , with statistical significance ( P<0.05 ) , with the in-crease of VPA concentration.Among the different VPA groups ,mutiple comparisons , the differences were statistically significant ( P <0.05 ) . Conclusions VPA could down -regulate the expression of Notch 1 , jagged1 and jagged2 in RPMI8226 cell, which may be one of the mechanism of action of VPA on the treatment of multi-ple myeloma.
