Analysis the expression change of hepatic glycogen in hepatocellular carcinoma in the mice induced by N-nitrosodiethylamine
10.13699/j.cnki.1001-6821.2015.10.026
- VernacularTitle:二乙基亚硝胺诱导小鼠原发性肝细胞癌过程中肝糖原的表达分析
- Author:
Qin-Chuan WU
1
;
San-Qiang LI
;
Jing-Feng HU
;
Zhi-Hui FU
;
Zhao MA
;
Hong-Mei HAN
;
Bo YANG
;
Kang-Xi DING
;
Xing-Ke YANG
Author Information
1. 河南科技大学 医学院 肝损伤与修复重点实验室
- Keywords:
N-nitrosodiethylamine;
hepatocellular carcinoma;
hepatic glycogen;
periodic acid-Schiff
- From:
The Chinese Journal of Clinical Pharmacology
2015;(10):851-854
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the change of hepatic glycogen in the process of hepatocellular carcinoma development in N -nitrosodiethy-lamine(DEN) -induced mice, and investigate the relationship between the hepatic glycogen content and the different stage of liver cancer . Methods There were forty clean and healthy male mice .They were di-vided in two groups ,the normal group ( n=3 ) and the model group ( n=37).After adapting environment in one week ,the group of normal mice were sacrificed and all the model animals were administrated by solution of DEN (30 μg? mL-1 ) in the carcinoma-induced period.Every three mice were sacrificed in the this group induced by DEN in 4 , 8 , 12 , 16 , 20 ,24 weeks(n=21).The obtained liver tissues divided in seven groups and embedded by paraffin were cut at thick piece .After staining with pe-riodic acid-Schiff ( PAS) the hepatic glycogen were observed .In order to obtain the mean optical density , samples of each group were disposed by the software Motic Images Advanced 3.2.Lastly.Analysis of variance ( ANOVA) test was followed by individual comparison by Student -New-man-Keul test using SPSS V16.0.Result The contents of hepatic gly-cogen in normal group , 8-week and 12-week groups were decreased successively and significantly , and the 16-week were least .The con-tents of hepatic glycogen in 16-week compared with 20-week and 24-week groups were increased successively and significantly . Conclusion In the process of development of hepatic carcinoma induced by DEN , the regular of hepatic glycogen′s change was decreasing gradually at the prior period ,and then the level of hepatic glycogen increased later .
