Effect of tetramethylpyrazine combined with cisplatin on expression of cascular endothelial growth factor and tumstatin of Lewis lung carcinoma in mice
10.13699/j.cnki.1001-6821.2015.10.025
- VernacularTitle:川芎嗪联合顺铂对肺癌中血管内皮生长因子和肿瘤抑素表达的影响
- Author:
Wei-Jia LIN
1
;
Xiu-Long ZHANG
;
Zhi-Hua ZHANG
;
Ping FENG
;
Jian-Hua TANG
Author Information
1. 河北北方学院 研究生部
- Keywords:
tetramethylpyrazine;
cisplatin;
lung cancer;
cascular endothelial growth factor;
tumstatin
- From:
The Chinese Journal of Clinical Pharmacology
2015;(10):847-850
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitiory effect and mechanism of tetramethylpyrazine in combination with cisplatin on Lewis lung carci-noma rats.Methods A transplanted tumor model of Lewis lung carcino-ma was establised.Then randomly divided into 4 groups: control group (0.9%NaCl,0.2 mL),cisplatin group (2 mg? kg -1,0.2 mL),tetram-ethylpyrazine group (100 mg? kg -1,0.2 mL), and combination group ( the doses as above ) , each 10 rats .Different treatments were served for 14 days,then all of rats were put to death.The tumor weight and in-hibitory rate were calculated .The tumor cells and vascular structure were observed under electron microscope .The expression of cascular endothe-lial growth factor(VEGF) and tumstatin were decteted by immunohisto-chemistry and Western blot .Results The weight was increased in all the groups.The control group was obviously fast than the other group . The combination group was obviously the slowest .The inhibition rate in combination group was significantly higher than the other groups .The tumstatin expression of combination group was obviously lower than the other three groups.The VEGF expression of combination group were sig-nificantly lower than the other three groups .The expression of VEGF had negative correlation with tumstatin ( P <0.05 ) .Conclusion Tetrame-thylpyrazine can effectively inhibit the growth of Lewis lung cancer trans-planted tumor , and has synergetic effect when combined with cisplatin . The mechanism might be that it can inhibit tumor angiogenesis by reducing VEGF expression , and promoting tumstatin expression.
