Analysis of characteristics of rpoB gene mutations associated with Mycobacterium tuberculosis resistance to rifampicin in Guizhou of China
10.13699/j.cnki.1001-6821.2015.10.021
- VernacularTitle:贵州地区结核分枝杆菌利福平耐药相关基因 rpoB突变特征分析
- Author:
Wei-Zheng OU
1
;
Ke-Wen LUO
;
Zheng-Hong CHEN
;
Jing CHEN
;
Yan WANG
;
Wan QIN
;
Jun MENG
Author Information
1. 贵阳市肺科医院检验科
- Keywords:
Mycobacterium tuberculosis;
rifampicin;
rpoB gene;
gene chip;
gene mutation
- From:
The Chinese Journal of Clinical Pharmacology
2015;(10):833-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study characteristics of rpoB gene mutations associated with Mycobacterium tuberculosis( MTB) resistance to rifampicin in Guizhou of China to discuss rapid screening methods of detecting MTB resistance to rifampicin.Methods MTB DNA of clinical samples were tested by fluorescent PCR .Low DNA concentration samples were excep-ted.All MTB DNA positive samples were tested by gene chip to screen rpoB gene mutation.The samples tested to be abnormal were further sequenced and aligned. Results MTB DNA of 3966 samples was positive.The positive rate was 22.72%(3966/17452 ).All of the 2738 samples were tested by gene chip , of which 552 samples were detected to have gene mutations and the rate was 20.16%( 552/2738 ) .Nine sam-ples of rpoB gene were tested abnormal .These 9 samples were confirmed to have nucleotide deletion , insertion, the same codon mutation of double base or multisite mutation .There were 26 types of gene mutations and mainly were at site 531 , site 526 , site 516 , site 511 , site 533 , site 513 and site 512 and the mutation rates were 56.23%, 18.09%, 9.67%, 8.61%, 4.57%, 1.76% and 0.18%, separately.Conclusion The characteristics of rpoB gene mutations associated with MTB resistance to rifampicin in Guizhou were basically the same as those in the literature reports.The gene chip can be used as the rapid screening method to detect MTB resistance to rifampicin in Guizhou .
