Expression change and significance of proliferating cell nuclear antigen in mice hepatocellular carcinoma
10.13699/j.cnki.1001-6821.2015.09.018
- VernacularTitle:增殖细胞核抗原在小鼠肝细胞癌发生中表达的变化及意义
- Author:
Zhao MA
1
;
San-Qiang LI
;
Zheng-Shun XU
;
Qin-Chuan WU
;
Jing-Feng HU
;
Zhi-Hui FU
;
Hong-Mei HAN
;
Hua-Jie LU
Author Information
1. 河南科技大学 医学院 肝损伤与修复分子医学重点实验室
- Keywords:
hepatocellular carcinoma;
proliferating cell nuclear antigen;
diethylnitrosamine
- From:
The Chinese Journal of Clinical Pharmacology
2015;(9):738-740
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic change and signifi-cance of proliferating cell nuclear antigen ( PCNA ) expression of mice which were induced into hepatocellular carcinoma ( HCC ) by diethylni-trosamine ( DEN ) .Methods Mice continuously induced cancer with DEN containing drinking water for 24 weeks .The mice were killed in the induction of cancer at different time points.The expression of PCNA protein and nucleic acid in mice liver with HCC were evaluated by Western blot and reverse transcription polymerase chain reaction,respec-tively.Results The expressions level of PCNA protein and nucleic acid in the liver tissue in mice after induced into HCC by DEN increased gra-dually.They reached a peaked at 24th week, and there was significant differences between them ( P<0.05 ).Conclusion There is a signifi-cant dynamic change of PCNA expression in the liver tissue in mice during the progression of HCC which were induced by DEN.It suggest that PCNA might play an important role in the incidence and development of HCC.
