Determination of imipenem in plasma of critically ill patients by high performance liquid chromatography method
10.13699/j.cnki.1001-6821.2015.08.018
- VernacularTitle:高效液相色谱法测定重症患者的亚胺培南血药浓度
- Author:
Nan GUO
1
;
Ying ZHAN
;
Hua SHAO
;
Wei-Lan LI
;
Feng YU
Author Information
1. 中国药科大学药学院
- Keywords:
imipenem;
high performance liquid chromatography ( HPLC);
plasma drug concentration;
critically ill patient
- From:
The Chinese Journal of Clinical Pharmacology
2015;(8):645-647
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a simple and rapid high -perfor mance liquid chromatography ( HPLC ) method for detecting the concen-tration of imipenem in critically ill patients.Methods The Alltima HP Hilic column (4.6 mm ×250 mm,5 μm) was used with acetonitrile -ammonium acetate ( 60∶40 ) as the mobile phase .The flow rate was 1 mL? min -1 and the column temperature was 35 ℃.The detector was operated at 298 nm.And then the specificity , lower limit of quantitation and standard curve , precision and recovery rate and stability of the method was investigated.Results Imipenem can be separated well , and the lowest limit of quantification was 0.5 μg? mL-1 .The regressive equation was A =4.9 ×10 3 C -216 ( r =0.999 5 ) , and the linearity range was 0.5-80.0 μg? mL-1 .The intra-day and inter -day RSD were both lower than 15%. The relative recovery rate ranged from 76.17%to 77.42%and the absolute recovery rate ranged from 93.90%to 104.66%.Conclusion This method is rapid, simple,accurate and stable , therefore it is suitable for the detection of imipenem in the human plasma , also can be used for the study on clinical pharmacokinetics of critically ill patients.
