Effects of Kangnaoye recipe preconditioning on nerve regeneration in rats CA1 area after cerebral ischemia and reperfusion
10.13699/j.cnki.1001-6821.2015.08.015
- VernacularTitle:康脑液预处理对局灶性脑缺血再灌注大鼠海马CA1区神经再生的影响
- Author:
Qing CHANG
1
;
Jian-Peng MA
;
YU-An ZOU
;
Qian XUE
Author Information
1. 河北北方学院 研究生院
- Keywords:
focal cerebral ischemia and reperfusion;
Kangnaoye recipe;
glial ibrillary acidic protein;
nerve regeneration
- From:
The Chinese Journal of Clinical Pharmacology
2015;(8):631-634
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of Kangnaoye recipe preconditioning on the nerve regeneration in rats CA 1 area after cerebral ischemia and reperfusion .Methods The 150 rats were randomly divi-ded into sham -operated group , model group , high -dose Kangnaoye group, middle-dose Kangnaoye group and low -dose Kangnaoye group (24, 12, 6 g? kg -1? d-1 ).The modle of cerebral ischemia and reper-fusion ( I/R) was induced by middle cerebral artery occlusion ( MCAO) and recanalization.Neurological symptom score were evaluated .HE stai-ning of the cells was used to observe the morphology changes in hippo-campus CA1 area.Glial ibrillary acidic protein ( GFAP ) protein and Nestin protein were observed by immunohistochemical method in CA 1 area.Results Compared with model group , neurological symptom score of Kangnaoye recipe preconditioning group were decreased and the result was significant ( P<0.01 ) .The groups pretreated with Kangnaoye had a lower expression of GFAP than the model group ( P<0.01 ) and the expression of the Nestin of groups pretreated with Kangnaoye were signifi-cantly higher than the model group (P<0.01) at the time of the 1,3,7 and 14 d after cerebral ischemia.Conclusion The Kangnaoye recipe preconditioning had protective effects on the pathological morphological changing ,it may be associated with promoting the neural stem cells regenera-tion and differentiation as well as inhibition the formation of fibrous scar .
