Molecular mechanism of Youguiyin inhibit the activity on the osteoclasts in rats Molecular mechanism of Youguiyin inhibit the activity on the osteoclasts in rats
10.13699/j.cnki.1001-6821.2015.07.021
- VernacularTitle:右归饮抑制大鼠破骨细胞作用的分子机制研究
- Author:
Wen-Xi DU
1
;
Jie-Fang HUANG
;
Jun-Jie CHEN
;
Li-Ming YIN
;
Chen-Jie XIA
;
Shu-Fang DUAN
Author Information
1. 浙江中医药大学 附属第一医院 骨科
- Keywords:
Youguiyin;
rheumatoid arthritis;
molecular mechanism;
osteoclast
- From:
The Chinese Journal of Clinical Pharmacology
2015;(7):557-559
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the molecular mechanism of You-guiyin inhibit the activity of rat osteoclasts through inhibiting Annexin A 2 ( AnxA2) and Atp61.Methods One hundred and five rats of collagen-induced arthritis ( CIA) were divided in to 7 groups:high, moderate and low doses Youguiyin groups , methotrexate group , lentiviruses ( +) group, lentiviruses ( -) group and model control group.And 15 rats without any treatment were included as blank group.Rats in the Yougui-yin groups were given Youguiyin intragastric.Rats in the methotrexate group were given methotrexate 1 μg· g -1 intragastric.Rats in the lenti-viruses (+) group were given 2.5 ×10 6 TU AnxA2 and Atp6 i lentiviru-ses double knee joints injection.Rats in the lentiviruses (-) group were given lentiviruses double knee joints injection.At the time of 8 weeks af-ter administration the rats were put to death.The serum level of tartrate resistant acid phosphatase -5b(TRACP-5b), the interleukin -1 beta ( IL-1β) , and knee joint bone density were evaluated.The change of the knee joint bone cortex in rats was evaluated by molybdenum target X-ray before and after administration.Results After 8 weeks, the IL-1βand TRACP -5b levels in CIA model group were much higher than that of blank group (P<0.05).IL-1βand TRACP-5b levels in Youguiyin, methotrexate, and lentiviruses ( +) groups were much lower than that of CIA model group ( P<0.05 ).The bone density in the Youguiyin and methotrexate groups were lower than the blank group ( P<0.05 ) but significant improve in the high dosages Youguiyin group than that of moderate and low dosage groups ( P<0.05 ) .After administration with Youguiyin , bone cortex is complete , bone mass distribution is uni-form according to X-ray in the high dosage group compared with the moderate and low groups.Conclusion In the process of osteoclasia in rats with rheumatoid arthritis , Youguiyin can inhibit the osteoclasia effects by regulate and con-trol the AnxA2 and IL-1βexpression.
