Effect of sevoflurane exposure on mRNA expression of N-methyl-D-aspartate receptor subunit in neonatal rat hippocampi
10.13699/j.cnki.1001-6821.2015.07.018
- VernacularTitle:七氟醚对新生鼠海马 N－甲基－D－天冬氨酸受体亚基 mRNA表达的影响
- Author:
Guo-Lin LU
1
;
Xiao-Lin CHEN
;
Su-Jing ZHANG
;
Xiao-Dan MAO
;
Liu LIAO
Author Information
1. 福建省妇幼保健院麻醉科
- Keywords:
anesthetics;
inhalation;
N-methyl-D-aspartate receptor;
in situ hybridization;
hippocampus
- From:
The Chinese Journal of Clinical Pharmacology
2015;(7):548-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sevoflurane on mRNA expression of N -methyl -D -aspartate ( NMDA ) receptor subunit in neonatal rat hippocampi.Methods Twenty-four SD rats were devided into 2 groups( n=12 each) via a random number table:sevoflurane group and control group.The 3.0% sevoflurane and oxy-gen inhalated for 6 h respectively.Rats were sacrificed at 24 h after inhalation of sevoflurane or oxygen.Hippocampi were removed to idendtify the mRNA expression of NMDA subunit and the ratio of NR2 B/NR2 A through in situ hybridization.Results Compared with control group, NR2BmRNA and the ratio of NR2B/NR2A increased, and NR2 AmRNA decreased significantly in sevoflurane group ( P<0.05 ).Conclusion Neurotoxicity of sevoflurane in immature hippocampi might be attributed to regulation of mRNA expression of NMDA subunit in neonatal rat.
