Study on the effect and mechanism of agmatine on blood brain barrier with cerebral ischemic reperfusion injury rats
10.13699/j.cnki.1001-6821.2015.07.017
- VernacularTitle:胍丁胺对脑缺血再灌注损伤大鼠血脑屏障的作用机制研究
- Author:
Li-Xia ZHENG
1
;
Xin-Miao XU
;
Xian-Bo LANG
;
Xiu-Li ZHANG
;
Mei-Zi YANG
Author Information
1. 滨州医学院药学院
- Keywords:
cerebral ischemic reperfusion;
brain edema;
blood brain barrier;
aquaporin;
agmatine
- From:
The Chinese Journal of Clinical Pharmacology
2015;(7):544-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of agmatine on brain ede-ma and examine the relation between blood brain barrier ( BBB) per-meability and aquaporin 4 ( AQP4 ) and aquaporin 9 ( AQP9 ) in rats with cerebral ischemic reperfusion.Methods Health male Sprague-Dawley rats were randomly divided into sham group , model group and test ( agmatine ) group.And 2 h after middle cerebral artery occlusion, giving agmatine ( 50 mg · kg -1 ) in test group or 0.9%NaCl in sham and model groups by intraperitoneal injection, respec-tively.Brain edema and the size of the brain infarct were assayed by brain water content and BBB permeability and 2,3,5 -triphenyltet-razoliumchloridstaining( TTC).The morphological changes of neurons were observed by hematoxylin and eosin staining and transmission electron microscopy.And the expression of AQP4 and AQP9 were assessed by Western -blotting.Results The BBB permeability de-creased significantly and the morphological changes of neurons were reduced significantly and the expression of AQP4 and AQP9 decreased significantly in rats with agmatine.Conclusion Agmatine reduce the injury of the BBB by decreasing the expression of AQP4 and AQP9 , and relieve the neuron injury induced by brain edema.
