Inhibition of long non-coding RNA metastasis -associated lung adenocarcinoma transcript 1 is associated with cisplatin resistance in human cervical carcinoma Hela cell
10.13699/j.cnki.1001-6821.2015.07.013
- VernacularTitle:肺腺癌转移相关转录子1与人宫颈癌 Hela细胞对顺铂耐药的相关性研究
- Author:
Xiu-Ling CHEN
1
;
Xiu-Hui CHNE
Author Information
1. 福建医科大学 第一临床医学院 妇产科
- Keywords:
long non-coding RNA;
human cervical cancer;
cisplatin;
drug resistance
- From:
The Chinese Journal of Clinical Pharmacology
2015;(7):530-531,543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the biological function of metastasis-associated lung adenocarcinoma transcript 1 ( MALAT1 ) associated with cisplatin resistance in Hela cell.Methods MALAT1 expression was know down by RNA interfere technology in human cervical carcinoma cell Hela.And the Hela cell was treated by 10 mg · L-1 cisplatin.The cell proliferation was tested by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 -H -tetrazolium bromide ( MTT ) array and cell cycle was tested by Flow cytometry assay.Results The cell proliferation was significant inhibit when decrease the expression in Hela cell ( P<0.05 ) . Which demonstrated that the inhibit MALAT 1 Hela cell was sensitive to cisplatin.And the G0/G1 cell cycle proportion was significant increased than the control group ( P <0.05 ).The migration ability in the si-MALAT1 Hela cell was significant decreased ( P<0.05 ).Conclu-sion Know down MALAT1expression can significant increase the cispla-tin sensitivity in human cervical cancer Hela cell.
