Study on the effective mechanisms of Hedysarum polybotrys polysacchcaide on delay the development of diabetic nephropathy in mice with diabetic nephropathy
10.13699/j.cnki.1001-6821.2015.07.012
- VernacularTitle:红芪多糖减缓糖尿病肾病小鼠病程进展的作用机制研究
- Author:
Xue-Yan QI
1
;
Zhi-Sheng JIN
;
Yan GUAN
;
Yu-Jiao WEI
;
Zhen-Ling ZHU
Author Information
1. 甘肃中医学院中医临床学院
- Keywords:
Hedysarum polybotrys polysacchcaide;
diabetic nephropathy;
p38 mitogen -activated protein kinase;
matrix metalloproteinases;
db/db mice
- From:
The Chinese Journal of Clinical Pharmacology
2015;(7):526-529
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protection effect of Hedysarum polybotrys polysacchcaide ( HPS) on early diabetic nephropathy ( DN) in db/db mice.Methods Fifty db/db male mice ( five weeks old ) were randomly divided into five groups:HPS ( low-dose , mid-dose , hing-dose) groups, telmisartan group, model group.Ten db/m mice ( five weeks old ) were employed as normal group.At the beginning of 6 weeks, were respectively given 200,100, and 50 mg · kg -1 HPS; 5 mg· kg-1 telmisartan.The mice in normol group and model group were given 0.9%NaCl by gavege.The 24 hours urine protein were measured after administration 8 weeks and kill the mice with remove blood and serum was isolated from the eye .And the related indexes were evaluated.Results After interventing 8 weeks,compared with model group , serum creatinine ( Scr) 24 hours urine proteinall in HPS and telmisartan groups decreased ( P<0.05 ).The levels of blood glucose , blood urea nitrogen ( BUN ) , the expression of p 38 mitogen -activated protein kinase ( p38 MAPK ) and matrix metalloproteinases ( MMPs ) mRNA all decreased , but only the high , middle doses groups of HPS were statisti-cally difference ( P <0.05 ) .Conclusion Which indicates that HPS could delay the development of DN by inhibiting the P 38MAPK,MMPs protein and mRNA expression in the glomerular mesangial cell membrane.
