Protective effect of Magnolia biondii Pamp on the acute alcoholic liver injury in mice and study its mechanisms
10.13699/j.cnki.1001-6821.2015.07.009
- VernacularTitle:辛夷对急性酒精性肝损伤小鼠的肝保护作用及机制研究
- Author:
Chuan-Feng HUANG
1
;
Hai-Xin WANG
;
Ai-Ying KANG
;
Guo-Liang ZHANG
;
Zhi-Feng JIAO
;
Ke DING
Author Information
1. 南阳医学高等专科学校 基础医学部
- Keywords:
Magnolia biondii Pamp;
alcoholic liver injury;
oxidative stress;
cytochrome P450 2 E1 mRNA
- From:
The Chinese Journal of Clinical Pharmacology
2015;(7):515-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Magnolia biondii Pamp ( MBP ) on hepatic antioxidant function and pathological histology for alcoholic liver injury in mice.Methods Fifty male KM mice were ran-domly divided into five groups as below:blank group , acute alcohol liver injury model group , MBP low -, medium -and high -dose groups (1.0, 2.0, 4.0 g· kg -1 ).The acute alcohol liver injury mouse model was set up by intragastric administration of alcohol once daily for 10 days.The activity of serum alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) , glutathione peroxidase ( GSH -PX ) activity and malondialdehyde ( MDA) content in liver were measured.The ex-pression of cytochrome P450 2E1 (CYP2E1) mRNA from the liver were determined by real-time fluorescent quantitative PCR.The pathological changes of liver were observed by light microscope with HE staining.Results Compared with the model group , MBP medium -and high-dose groups have little pathological damage of liver.The levels of ALT, AST, MDA and CYP2E1 mRNA were significant decreased while GSH-PX was significant increased.Conclusion MBP have a signifi-cant protective effect on acute alcohol liver injury in mice , which exerted its beneficial effects mainly through inhibiting oxidative damage and de-creased the expression of CYP 2E1 mRNA.
