Simultaneous determination of sodium danshensu, protocatechualdehyde and caffeic acid, rosmarinic acid and salvianolic acid A in the plasma of rats by HPLC-MS/MS method
10.13699/j.cnki.1001-6821.2015.04.018
- VernacularTitle:建立同时测定大鼠血浆中丹参素钠与原儿茶醛和咖啡酸、迷迭香酸和丹酚酸 A含量的液质联用方法
- Author:
Ying-Ming ZHENG
1
;
Qi QIU
;
Sa-Li CAO
;
Jing FU
;
Xing-Bin YIN
;
Wen-Fang LIU
;
Ke-Xu YANG
;
Jing-Lin CAO
;
Jian NI
;
Yang LIN
Author Information
1. 北京市心肺血管疾病研究所 -首都医科大学附属北京安贞医院药事部
- Keywords:
sodium danshensu;
protocatechualdehyde;
caffeic acid;
rosmarinic acid;
salvianolic acid A;
HPLC-MS/MS;
blood concentration
- From:
The Chinese Journal of Clinical Pharmacology
2015;(4):297-299,303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC-MS/MS method to deter-minate the concentration of sodium danshensu, protocatechualdehyde and caffeic acid, rosmarinic acid and salvianolic acid A in rat plasma. Methods The analytes were extracted by protein precipitation.Synergi Hydro-RP 80 A (2 mm ×150 mm, 4 μm) column was used.An gra-dient mobile phase consisting of acetonitrile-0.1% formic acid in wa-ter, 0~2 min, 10%~60% acetonitrile,2~3 min, 60%~95%aceto-nitrile,3~6.5 min, 95% acetonitrile,6.5~7 min, 95%~10% aceto-nitrile,7~12 min,10%acetonitrile,flow velocity was 0.4 mL? min -1 . After electrospray ionization ( ESI ) , multiple reaction ion monitoring ( MRM ) mode was adopted to detect the negative ions.Results The linearity range were 10 -1000,5 -500,2 -200,5 -500,10 -1000 ng? mL-1 respectively.The lower limit of quantitation of drugs were 10, 5,2,5,10 ng? mL-1 .Intra and inter precisions ( RSD) were less than 8.31%and 12.73%.Extraction recoviries for sodium danshensu, proto-catechualdehyde, caffeic acid, rosmarinic acid and salvianolic acid A were both greater than 50%.Conclusion This method was simple, high specificity, good stability with good repeatability.It could be used to determinate the plasma concentration of sodium danshensu, protocatechualdehyde, caffeic acid, rosmarinic acid and salvianolic acid A correctly and coinstantaneously.
