Determination of taurine in human plasma by high performance liquid chromatography
10.13699/j.cnki.1001-6821.2015.04.016
- VernacularTitle:高效液相色谱法测定人血浆中牛磺酸的浓度
- Author:
Hong-Xiang TAI
1
;
Wei-Zhen GAO
;
Xiao-Jing FAN
;
Qin LI
;
Jian-Jie JIAO
;
Jian-Shi LOU
;
Cai-Li ZHANG
Author Information
1. 天津医科大学药理学教研室
- Keywords:
high performance liquid chromatography;
taurine;
plasma concentration
- From:
The Chinese Journal of Clinical Pharmacology
2015;(4):289-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chroma-tography ( HPLC ) method to determine taurine in human plasma. Methods Former derivatization method by DNFB pillar, with gradient elution of each component was adopted.The compounds were separated on a Kromasil C18 column, and mobile phase A: 0.05 mol? L-1 CH3COONa solution ( pH 6.5 ) , mobile phase B: acetonitrile -water (1∶1). The flow rate was 1 mL? min-1 , column temperature was 30 ℃, and the detection wavelength was 360 nm.Results The assay had the calibration range from 3.12 to 400.00μg? mL-1 ( r>0.999 9 ) , and the intra-day RSD and inter-day RSD were all lower than 5%. The lowest limit of quantification was 3.12 μg? mL-1 for taurine. Conclusion The method is simple, accurate, sensitive and specific for the determination of taurine in human plasma.
