Effect of fosfomycin combined with levofloxacin on Acinetobacter baumannii biofilm
10.13699/j.cnki.1001-6821.2015.04.012
- VernacularTitle:磷霉素联合左氧氟沙星对鲍曼不动杆菌生物膜的体外研究
- Author:
Kui YAN
1
;
Han-Chun WEN
;
Yi-Qiang CHEN
;
Hong-Jie LIANG
;
Meng LI
;
Li MIN
Author Information
1. 广西医科大学 第一附属医院急诊科
- Keywords:
Acinetobacter baumannii;
biofilm;
fosfomycin;
levofloxacin;
bactericidal effect
- From:
The Chinese Journal of Clinical Pharmacology
2015;(4):276-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the in vitro destructive effect of fos-fomycin ( FOS ) on the biofilm of Acinetobacter baumannii and the synergistic antibacterial activity in combination with levofloxacin ( LFX) .Methods The biofilm model was established by clinical isolates of Acinetobacter baumannii -48876 as the study strain.The minimum inhibitory concentrations ( MIC ) of FOS and LFX were measured by doubling dilution. The viable count of biofilm carrier were determined by continuous dilution method and the biofilm quanti-tation by crystal violet staining method.Data were analyzed by single factor analysis of variance ( One-Way ANVOA).Results When the biofilm being reacted with antibiotic for 24 hours, the quantitation of biofilm showed the absorbances of FOS group and FOS plus LFX group were significantly less than that of the control group ( P<0.01 ) .While there was no statistical difference found in absor-bance value in LFX group and the control group.At the 4 th , 8 th and 24 th hour, the number of bacteria in biofilm of FOS plus LFX group was significantly less than that in the control group ( P<0.01 ) , While there was no statistical difference found between FOS or LFX group and control group.Conclusion FOS can destroy the biofilm formed by Acinetobacter baumannii, and the Acinetobacter baumannii in the biofilm carrier could be significantly reduced while plus LFX.
