Determination of hydromorphone concentration in human plasma by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2015.03.017
- VernacularTitle:高效液相色谱-质谱联用法测定人血浆中氢吗啡酮的浓度
- Author:
Heng-Yan QU
1
;
Guang-Tao HAO
;
Xue-Yi CHEN
;
Hong-Zhi GAO
;
Rui-Hua DONG
;
Yuan-Yuan LI
;
Xiao-Fang WANG
;
Ze-Yuan LIU
;
Hong ZHANG
Author Information
1. 军事医学科学院附属医院 临床药理室
- Keywords:
HPLC-MS/MS;
hydromorphone;
pharmacokinetic
- From:
The Chinese Journal of Clinical Pharmacology
2015;(3):216-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the mass concentration in human plasma hydromorphone by established HPLC -MS/MS method.Methods The treatment of plasma with solid-phase extraction, selec-tion Kromasil 100 -5 SIL, E97915 column with 95% acetonitrile -5 mmol· L-1 ammonium acetate as the mobile phase gradient elution with positive ion, multiple reaction monitoring mode mass concentration of the sample was measured.Results The plasma samples, hydromor-phone in good ( r >0.999 8 ) 0.05 -10.00 ng · mL-1 linear relation-ship, the minimum detectable concentration was 0.05 ng· mL-1.Blood samples day and inter-day RSD were less than 15%, the average recovery >70%, and stability were good.Conclusion The method is simple, rapid, sensitive and accurate, specific, kinetics in vivo pharma-cokinetics of hydromorphone apply.
