Determination the concentration of citalopram and its metabolites in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry
10.13699/j.cnki.1001-6821.2014.03.016
- VernacularTitle:超高效液相串联质谱法测定大鼠西酞普兰及其代谢物的血药浓度
- Author:
Chun-Mei WU
1
;
Shuang-Hu WANG
;
Guo-Xin HU
;
Yun-Fang ZHOU
Author Information
1. 丽水市人民医院 药学部
- Keywords:
citalopram;
plasma concentration;
pharmacokinetic;
ultra performance liquid chromatography-tandem mass spectrometry
- From:
The Chinese Journal of Clinical Pharmacology
2015;(3):212-215
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an ultra performance liquid chroma-tography -tandem mass spectrometry method for the determination the concentration of citalopram and its metabolites ( citalopram N-oxide and N -desmethylcitalopram ) in rat plasma.Methods Plasmas were extracted with ethyl acetate.An ACQUITY UPLC BEH C18 ( 50 mm × 2.1 mm, 1.7 μm) column was used as the stationary phase.The mobile phase was consisted of acetonitrile and 0.1% formic acid water with gradient elution pumped at a flow rate of 0.4 mL· min-1.The analytes were detected with positive electrospray ionization in multiple reaction monitoring ( MRM ) mode and tramadol was used as internal standard.Results Excellent linear calibration curves of citalopram, N-desmethyl-citalopram and citalopram N-oxide were obtained in the concentration range of 0.1-10.0, 0.1-10.0 and 0.01-2.00 ng· mL-1 ( r=0.999 6, 0.999, 0.999 6).The lower limit of quantification were 0.05, 0.05 and 0.01 ng · mL-1.The average recovery was 96.09%-105.33%.The intra and inter day relative standard deviations were all less than 8.69%. Conclusion The method is simple, rapid and sensitive, which is suit-able for pharmacokinetics study of citalopram and its metabolites in rats.
