Research of rapid mass spectrum identification of Acinetobacter baumannii
10.13699/j.cnki.1001-6821.2015.02.009
- VernacularTitle:质谱法对鲍曼不动杆菌的快速鉴定研究
- Author:
Ya-Ping HANG
1
;
Long-Hua HU
;
Hong WANG
;
Chang-Xiu NING
;
Qiao-Shi ZHONG
;
Xiao-Yan HU
;
Bai-Ling ZHANG
;
Nan ZHANG
;
Xiao-Zhong WANG
Author Information
1. 南昌大学 第二附属医院 检验科
- Keywords:
Acinetobacter baumannii;
volatile metabolite;
surface desorp-tion atmospheric pressure chemical ionization mass spectrometry
- From:
The Chinese Journal of Clinical Pharmacology
2015;(2):110-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the rapid identification of Acineto-bacter baumannii by detecting specific volatile metabolites of Acinetobacter baumannii through surface desorption atmospheric pressure chemical ioni-zation mass spectrometry ( SDAPCI-MS).Methods Ten clinical isola-ted Acinetobacter baumannii were resuscitated and vaccinated to blood agar plates at 37 ℃incubation and culturing.SDAPCI-MS was adopted to detect volatile metabolites of Acinetobacter baumannii by different cul-turing time.Results Compared with blank culture medium , the pecu-liar volatile metabolites were detected ,the characteristic peaks are mass -to-charge ratio 60 and 118.Basically the same volatile metabolites of Acinetobacter baumannii were generated by different culturing time.Con-clusion The rapid identification of Acinetobacter baumannii through peculiar volatile metabolites is hopefully applied by SDAPCI -MS.
