Modified two vessel blocking method of acute global ischemia model in rats
10.13699/j.cnki.1001-6821.2015.01.021
- VernacularTitle:大鼠双侧颈总动脉阻断法致全脑缺血模型的改良研究
- Author:
Zheng-Qi LI
1
;
Chen-Yu FAN
;
Hong-Yi HOU
;
Zheng WANG
;
Sang-Tian HUANG
;
Yuan GAO
;
Hai-Ling QIAO
Author Information
1. 郑州大学 临床药理研究所
- Keywords:
global ischemia model;
modified;
death rate;
behavior;
his-topathological change
- From:
The Chinese Journal of Clinical Pharmacology
2015;(1):70-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To adapt to the acute cerebral ischemia re-search , we modified the permanent bilateral common carotid artery occlu-sion (2VO) rat model.Methods Global ischemia models were estab-lished by permanent ligation of bilateral common, internal, external ca-rotid arteries (6VO) and the 2VO method respectively.The sham group were done simultaneously.Then the differences among three groups in mortality, behavioral score tests,pathological morphology, the expression of inflammatory factor by immunohistochemical method, and the percen-tage of cell apoptosis were compared.Results Twenty-four hours after operations, the mortality of 6VO group ( 40% ) was obviously higher than 2VO group ( 0 ) .The results of behavioral score, necrosis rate of neurons, the expression of inflammatory factors and cell apoptosis in hippocampus CA1 area in 6VO group were significantly higher than 2VO group and sham group ( P <0.05 ) .Conclusion The modified 6VO model is an ideal way to observe acute global ischemic injury in rats.
