Experimental study of Bushen Yiqi recipe on slowing the aging progress of skeletal muscle
10.13699/j.cnki.1001-6821.2015.01.019
- VernacularTitle:补肾益气方药延缓骨骼肌衰老的实验研究
- Author:
Hua-Ping XU
1
;
Guo-Qin JIN
;
Zhang-Bin GONG
;
Xiang-Ping KANG
Author Information
1. 上海中医药大学
- Keywords:
Bushen Yiqi;
aging;
D-galactose;
skeletal muscle;
antioxidant capacity
- From:
The Chinese Journal of Clinical Pharmacology
2015;(1):63-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Bushen Yiqi recipe on aging rats′ exercise capacity, morphology and oxidation resis-tance.Methods Male sprague dawley rats were divided into normal group, model group, traditional Chinese medicine group, methandienone group.The rats in the latter three group were received a subcutaneous in-jection of 10%D-galactose with 0.1 mg? g -1? d-1 for 10 weeks.The rats in traditional Chinese medicine group and methandienone group were given medicine by gavage .The dosage is adults′7 times of per kilogram of body weight and equivalent dose respectively.The experiment were las-ted for 10 weeks.Then rats′exercise capacity were tested by treadmill. The Morphological structure of the skeletal muscle were observed by elec-tron microscope.Rats′advanced glycosylated end products( AGEs) were tested by Elisa method.Rats′superoxidedismutase ( SOD) and malondi-aldehyde ( MDA) content in blood serum were tested by spectrophotome-try.Results Compared with normal group, the rats′exercise capacity, the mitochondria number and volume of rats′skeletal muscle, the serum SOD activity, the thymus index and spleen index in model group dropped significantly.The serum AGEs and MDA content increased significant-ly.Compared with model group, the exercise capacity, the mitochondria number and volume of rats′skeletal muscle, the thymus index in traditional Chinese medicine group and methandienone group inceased obviously.The serum MDA content decreased obviously.Conclusion Bushen Yiqi recipe can slow the aging progress of skeletal muscle through improving the model rats′degenerative changes of the above-mentioned indexes.
