Effects of contrast agent on neutrophil gelatinase-associated lipocalin in blood and Bax mRNA in kidney of rats with unilateral ureteral obstruction
10.13699/j.cnki.1001-6821.2015.01.013
- VernacularTitle:造影剂对单侧输尿管梗阻大鼠血中性粒细胞明胶酶相关脂质运载蛋白及肾 Bax mRNA的影响
- Author:
Qiang XIA
1
;
Chun-Xiao LIU
;
Xiao-Bin ZHANG
;
Zhi-Yong ZHONG
;
Xia ZHENG
Author Information
1. 南方医科大学 珠江医院 泌尿外科
- Keywords:
contrast media;
ureteral obstruction;
hydronephrosis;
neutrophil gelatinase -associated lipocalin;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2015;(1):41-43,47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effects of contrast agent on neutrophil gelatinase-associated lipocalin( NGAL) in blood and Bax mRNA in kid-ney of rats with unilateral ureteral obstruction.Methods SD rats were randomly divided into sham,control and contrast agent groups(test).The rats of control and test groups were prepared refolding complete obstruc-tion of the left ureter.The hological changes was deteceted in the kidneys and level of NGAL was measured in rats serum.The bular cell apoptosis was observed and expression of Bax mRNA was measured in kid-neys.Results After the injection of the contrast agent on one day, the volume of rat kidneys, renal parenchymal thickness and weight were sig-nificantly different between left and right kidneys.Compared with control group,the level of NGAL and Bax mRNA in the test group was signifi-cantly higher (P<0.05).The first day and the first three weeks after in-jection, the level of NGAL and Bax mRNA of test group compared with control group was significantly increased ( P<0.05).After the first three weeks,the left renal tubular cell apoptosis in control group compared with test group was significantly higher ( P<0.05 ) .Conclusion Contrast agents have a certain injury in rat kidneys with unilateral hydronephrosis, and apoptosis of renal tubular cell mediated by Bax may be an important mechanism.
