Study on the transport mechanisms of levofloxacin in a model of gastric mucosa in vitro
10.13699/j.cnki.1001-6821.2015.01.008
- VernacularTitle:左氧氟沙星在胃黏膜体外模型中的转运机制研究
- Author:
Shan-Shan ZHANG
1
;
Yong-Mei HU
;
Lei ZHANG
;
Xing-Ping HU
;
Jian-Ming XU
Author Information
1. 安徽医科大学 第一附属医院
- Keywords:
levofloxacin;
gastric mucosa;
Ussing chamber;
HPLC;
transport;
P-glycoprotein
- From:
The Chinese Journal of Clinical Pharmacology
2015;(1):25-27,30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the transport characteristics of levofloxacin.Method A model was established in vitro by gastric muco-sa combined with Ussing chamber for studying the gastric mucosa trans-port mechanisms of levofloxacin.The effects of concentration of donor so-lutions, direction, pH and P -glycoprotein ( P -gp ) inhibitor on the transport of levoflo-xacin were investigated.The concentrations of levo-floxacin were determined by high performance liquid chromatography ( HPLC) and the transport parameters such as apparent permeability co-efficient ( Papp) was calculated.Results The transport of levofloxacin obeyed a passive diffusion in a pH-dependent manner, and significant differences between the Papp of two directions.The P-gp inhibitor vera-pamil enhanced the transport amount of levofloxacin.Conclusion The reasults indicated that carrier-mediated transport manner might play a major role in the transport of levofloxacin in this model in vitro and P-gp probably participated in the transport of levofloxacin.
