Study on the multilocus sequence typing of carbapenem resistant Klebsiella pneumonia
10.13699/j.cnki.1001-6821.2015.01.006
- VernacularTitle:碳青霉烯类耐药肺炎克雷伯菌多位点序列分型研究
- Author:
Chang-Xiu NING
1
;
Long-Hua HU
;
Hong WANG
;
Qiao-Shi ZHONG
;
Ya-Ping HANG
;
Xiao-Yan HU
;
Xiao-Zhong WANG
;
Jian-Qiu XIONG
Author Information
1. 南昌大学 附属第二医院 检验科
- Keywords:
Klebsiella pneumoniae;
carbapenemase;
multiple drug resistance;
multilocus sequence typing
- From:
The Chinese Journal of Clinical Pharmacology
2015;(1):17-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular epidemiology of car-bapenem resistant Klebsiella pneumonia( CRKP) from four teaching hos-pitals in Nanchang by multilocus sequence typing ( MLST ) .Methods Twenty-nine imipenem and/or meropenem resistant Klebsiella pneumoni-a isolates were totally collected from four teaching hospitals in Nanchang from Jan 2011 to June 2012.The polymerase chain reaction( PCR) am-plifications of drug resistant genes were performed.The molecular genetic of CRKPs were analyzed by MLST.The population structure of CRKPs was evaluated by eBURST with the results of MLST.Results Twenty-nine CRKPs were successfully divided into 16 sequence types ( STs ) by MLST.The main types were ST11 and ST15 (5/29 each); followed by ST395 ( 3/29 ); ST334, ST273, ST147 ( 2/29 each ); ST1128, ST42, ST1031,ST764,ST629 and ST37(1/29 each).It is worth nothing that 4 new STs have been confirmed by the PubMlst database and named ST1369,ST1411,ST1412,ST1413.Among 2 ST groups, each ST group respectively included 2 STs ( ST395 and ST1369, ST147 and ST273 ) , the other 12 STs were single type, which ST11 ,ST15 and ST395 belongs to the CC258 clonal complex. Conclusion Gene polymorphism was existed on CRKPs of this region and the main types of 29 experimental strains were ST11 and ST15, and ST15 has been briefly local disseminated.
