Study on the best choice of adjuvant on passive cutaneous anaphylaxis in rats
10.13699/j.cnki.1001-6821.2014.12.012
- VernacularTitle:大鼠被动皮肤过敏性实验中最佳佐剂的选择研究
- Author:
Bao-Qin JING
1
;
Jin-Xiao ZHANG
;
Lei WANG
;
Rui DUAN
;
Zong-Peng ZHANG
Author Information
1. 天津药物研究院 天津市新药安全评价研究中心
- Keywords:
passive cutaneous anaphylaxis;
adjuvant;
aluminium hydro-xideb;
pertussis bacteria liquid;
diphtheria - pertussis -tetanus combined vaccine vaccine
- From:
The Chinese Journal of Clinical Pharmacology
2014;(12):1114-1116
- CountryChina
- Language:Chinese
-
Abstract:
Objective To get the best choice of adjuvant by researching the influence of the adjuvant whether it is added or not on passive cutane-ous anaphylaxis test and the distinction of different adjuvant which is used on passive cutaneous anaphylaxis ( PCA) test.Methods With ovalbu-min (OVA) as allergens, SD rats were injected three different adjuvant , pertussis bacteria liquid ( 0.25 mL per animal ) , aluminium hydroxide ( 0.5 mL per animal ) , diphtheria-pertussis-tetanus combined vaccine ( DPT) ( 0.25 mL per animal ) on PCA test.The skin allergic reaction was investigated.Result Each group can cause allergic reaction with different degrees.The incidence of allergic reaction in adjuvant group was obviously higher than blank group.According to the incidence and severity of allergic reaction , the result from large to small is DPT vac-cine , pertussis bacteria liquid , aluminium hydroxide.The number of ani-mals with allergic reaction in female rats is higher than the number in male rats.Conclusion Allergic reaction using different adjuvant on PCA test was different .Taking comprehensive consideration of different adjuvant in different factors , such as purchase , transportation , price and the sensitization effect , aluminium hydroxide can be the best choice on PCA test.
