Toxic effects of 2-dodecenoic acid on human hepatoma cells
10.13699/j.cnki.1001-6821.2014.12.010
- VernacularTitle:2－十二烯酸对人肝癌细胞毒性作用研究
- Author:
Fa-Rong YU
1
;
Xiu-Zhen LIAN
;
Ming-Ren XIE
;
Zuo-Ping LI
;
Deng-Lou LI
Author Information
1. 甘肃政法学院 甘肃省证据科学技术研究与应用重点实验室
- Keywords:
2 -dodecenoic acid;
human hepatoma cell;
toxicity;
cell cycle;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2014;(12):1108-1110,1123
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the toxicity of 2-dodecenoic acid on human hepatocarcinoma cells.Methods The present study is to in-vestigate the toxic effects of 2 -dodecenoic acid on human hepatoma tumor cells by using trypan blue staining , MTT assay , and flow cytometry analysis.Results After cancer cells were treated with concentrations of 0.1 , 0.3 ,0.9 mg· L-1 2-dodecenoic acid at 6 h, 12 h, 18 h, 24 h, and 48 h, respectively.Compared to the control group , 48 h after treat-ment, the inhibition rates of the hepatoma cells proliferation in treatment group were increased by 45.46%, 67.16%, 79.18%at the above three concentrations , respectively ( P<0.01 ).Flow cytometry analysis showed that the apoptosis rates after the 2-dodecenoic acid treatment were in-creased by 14.8%, 20.2% and 24.2% over the control group.Conclusion These results indicated that the 2-dodecenoic acid has a significant toxic effect on human hepatoma cells.
