Expression analysis of a disintegrin and metalloprotease 8 during hepatocellular carcinoma induced by diethylnitrosamine in mice
10.13699/j.cnki.1001-6821.2014.12.009
- VernacularTitle:解整合素－金属蛋白酶8在二乙基亚硝胺诱导的动物肝细胞癌过程中的表达分析
- Author:
San-Qiang LI
1
;
Xu-Hong CUI
;
Hong-Ye MENG
;
Zhao MA
;
Hong-Mei HAN
Author Information
1. 河南科技大学 医学院 肝脏损伤与修复分子医学重点实验室
- Keywords:
diethylnitrosamine;
a disintegrin and metalloprotease 8;
hepa-tocellular carcinoma;
expression analysis
- From:
The Chinese Journal of Clinical Pharmacology
2014;(12):1105-1107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expressed dynamic change of a dis-integrin and metalloprotease 8 ( ADAM8 ) during hepatocellular carci-noma ( HCC ) induced by diethylnitrosamine ( DEN ) in mice.Methods Seventy mice were randomly divided into two groups:nor-mal group ( n=10 ) and experimental group ( n=60 ).The mice in experimental group were fed daily with 30 μg · mL -1 DEN in drin-king water for 24 weeks.Ten mice were respectively sacrificed at 4 , 8 , 12 , 16 , 20 and 24 weeks, then the livers were taken from the mice and divided into two sections.One was used to detect the occurrence of liver cancer by HE staining, the other was used to detect the ADAM8 expression by Western blot during HCC induced by DEN in mice.The normal control mice were also detected as experimental mice.Results HCC model was successfully induced at 24 weeks after DEN induction.The expression of ADAM8 was gradually increased and reached the peak level at 24 weeks after DEN induc-tion.Conclusion ADAM8 were remarkably differently expressed during HCC induced by DEN in mice.ADAM8 could play important roles in promoting the occurrence and development of HCC.
