Comparing the API and VITEK2 in identifying VanC type Enterococcus

VernacularTitle:API和VITEK2诊断VanC型肠球菌的准确性研究
Author: Qiang DAI ¹ ; Feng XUE ; Si-Jing WANG ; Chun GAO ; Bo ZHENG
Author Information

1. 北京大学第一医院临床药理研究所
Keywords: VanC type Enterococcus; API; VITEK2; vancomycin resis-tant; motility test
From: The Chinese Journal of Clinical Pharmacology 2014;(12):1096-1097,1104
CountryChina
Language:Chinese
Abstract: Objective To compare the API and VITEK2 in identifying VanC type Enterococcus.Method A total of 50 VanC type Entero-coccus isolates from 2005~2011 were investigated.We performed the API and VITEK2 test to identified the VanC type Enterococcus respec-tively.API and VITEK2 results combining with the motility test was compared with molecular biology methods to determine the accuracy of API and VITEK2.The MIC of vancomycin and tecoplanin for the strains was performed by agar dilution method.Results Twenty -one and 15 strains of VanC phenotype Enterococcus were identified by the API and VITEK2 test respectively.Compared with molecular bio-logy methods, the accuracy rate was 28.6% and 86.7% respective-ly.Combined with motility test, the accuracy rate of API and VITEK2 reached 100%.Conclusion The VITEK2 had a higher accuracy rate in identifying VanC phenotype Enterococcus than API.API/VITEK2 method combined with bacterial motility test can provide an accurate bacteriological diagnosis for the patients.