Improved Antibiotic Susceptibility Test of Orientia tsutsugamushi by Flow Cytometry Using Monoclonal Antibody.
- Author:
Mi Jeong KIM
1
;
Mee Kyung KIM
;
Jae Seung KANG
Author Information
- Publication Type:Original Article
- Keywords: Orientia tsutsugamushi; Doxycycline; Antibodies, Monoclonal; Flow Cytometry; Antibiotic Susceptibility; Microbial Sensitivity Tests
- MeSH: Orientia tsutsugamushi/*drug effects/growth & development; Microbial Sensitivity Tests/*methods; Flow Cytometry/*methods; Antibodies, Monoclonal/*immunology
- From:Journal of Korean Medical Science 2007;22(1):1-6
- CountryRepublic of Korea
- Language:English
- Abstract: Orientia tsutsugamushi causes scrub typhus, which is endemic in many countries in the Asia-Pacific region including Korea. Recent emergence of doxycycline-resistant strains from Thailand has underlined the importance of the susceptibility tests of O. tsutsugamushi to antibiotics. To improve the flow cytometric technique for the susceptibility test, we applied a monoclonal antibody (MAb) in the quantification of O. tsutsugamushi. With using MAb FS15, we determined the doxycycline susceptibility of two strains, Boryong and AFSC-4 strain which is reported to be doxycycline-sensitive and resistant, respectively. The growth of both strains was inhibited to below 10% of the control in the presence of 0.1 microgram/mL or higher concentrations of doxycycline. We suggest that our approach is more quantitative and reproducible than the conventional microscopic methods.
