Effect of paeoniflorin on toxicity and protection mechanism of oxidative injury of L02 cell induced by ethanol
10.13699/j.cnki.1001-6821.2014.11.016
- VernacularTitle:芍药苷对乙醇诱导 L02细胞的毒性和氧化损伤保护机制的影响
- Author:
Min HUANG
1
;
Hong-Bing BO
;
Xi WANG
;
Xiao-Bin WANG
;
Xue-Mei SU
;
Zhen XIANG
;
Xue-Wen GAN
;
Zhi-Yan SHI
Author Information
1. 解放军第181医院 整形外科
- Keywords:
paeoniflorin;
ethanol;
L02 cell;
cytochrome P450 2E1;
lipid peroxidation
- From:
The Chinese Journal of Clinical Pharmacology
2014;(11):1030-1032,1038
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of paeoniflorin ( PF) on cytochrome P450 2E1( CYP450 2E1) and lipid peroxidation in L02 cell induced by ethanol.Methods L02 cell model was established by 75 mmol · L-1 ethanol.After treated with paeoniflorin, superoxide dismutase ( SOD) and malondialdehyde ( MDA) levels in the cell culture supernatant were detected by biochemical analysis.The expressions of CYP450 2E1 in L02 cell were observed by RT-PCR and immunocyto-chemistry.Results Compared with the control group, serum SOD levels in model group were significantly decreased, while MDA levels were elevated ( P <0.05 ).The levels of CYP450 2E1 mRNA and protein were significantly increased in model group (P<0.05).However, com-pared with the model group, SOD levels in the cell culture supernatant of PF group were much higher, while MDA levels were decreased, and the levels of CYP450 2E1 mRNA and protein were significantly decreased in PF group ( P<0.05 ).Conclusion The effect of PF on the improve-ment of L02 cell damaged by ethanol and reducing lipid peroxidation could be related with the regulation on the expression of CYP450 2 E1.
