Effects of Gypsophila oldhamiana gypsogenin on growth and apoptosis of human colon cancer HT-29 cells
10.13699/j.cnki.1001-6821.2014.11.013
- VernacularTitle:霞草苷对人结肠癌HT－29细胞生长和凋亡的影响
- Author:
Gui-Hong TIAN
1
;
Ying QIN
;
Ling ZHOU
;
Ying ZHONG
;
Yan-Ling MU
Author Information
1. 山东省医学科学院 药物研究所
- Keywords:
colon cancer;
Gypsophila oldhamiana gypsogenin;
cell apoptosis;
caspase-3;
Bcl-2 associated X protein
- From:
The Chinese Journal of Clinical Pharmacology
2014;(11):1020-1022
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Gypsophila oldhami-ana gypsogenin( GOG) on induction of apoptosis in human colon cancer HT-29 cells.Methods The growth and apoptosis of HT -29 cells treated with 5,10,20,40 μmol· L-1 GOG are investigated by MTT assay and flow cytometry.The mRNA expression of Caspase-3 and Bax was measured by real-time fluorescence quantitative RT-PCR.Results GOG inhibited the proliferation of HT -29 cell line in a dose -and time-dependent manner. Cell apoptosis rate increase obviously.Caspase-3 and Bax mRNA expression quantity increase gradually, assumes the obvious density dependence.Conclusion GOG could inhibit HT-29 cell proliferation through inducing cell apoptosis in vitro.The mechanism might be that GOG could enhance the level of Bax and activate the pathway of Caspase-3.
