Expression of a disintegrin and metalloprotease 8 during the drug -induced liver in mice
10.13699/j.cnki.1001-6821.2014.10.017
- VernacularTitle:解整合素-金属蛋白酶8在小鼠药物性肝损伤中的表达
- Author:
San-Qiang LI
1
;
Xu-Hong CUI
;
Lan-Ze YANG
;
Zhao MA
;
Hong-Mei HAN
Author Information
1. 河南科技大学 医学院 肝脏损伤与修复分子医学重点实验室
- Keywords:
acetaminophen;
a disintegrin and metalloprotease 8;
drug -induced liver injury;
protein expression
- From:
The Chinese Journal of Clinical Pharmacology
2014;(10):926-928
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expressed dynamic change and roles of a disintegrin and metalloprotease 8 (ADAM8) during drug -induced liver injury induced by acetaminophen (AAP).Methods Mice were randomly divided into two groups:normal group and experimental group. The mice in experimental group were respectively drawn blood by remo -ving the eyeballs and serum was separated to detect the activity of aspar -tate aminotransferase (AST) and alanine aminotranferease (ALT) at 6, 24, 42 and 72 h after intraperitoneal injection with 550 mg? kg-1 AAP. The activity of serum AST and ALT in the mice of normal group was also detected.The expression of hepatic ADAM8 at protein and mRNA levels was detected by Western blot and RT -PCR in the mice of normal group and experimental group at different time points after AAP injection . Results Serum AST and ALT activity was first increased and then decreased after AAP injection in the mice.The expression of ADAM8 were significantly up -regulated (P <0.05) at 6 h, reached peak level at 24 h (P <0.05).Then gradually down -regulated and recovered near the normal level at 72 h after AAP injection.Conclusion ADAM8 were remarkably differently expressed during drug -induced liver injury induced by AAP, which may play an important role in promoting liver damage during drug -induced liver injury induced by AAP.
