Analysis on the resistance of clinically cefoxitin -non -susceptible Klebsiella pneumoniae isolates and prevalence of β-lactamase
10.13699/j.cnki.1001-6821.2014.10.015
- VernacularTitle:头孢西丁不敏感肺炎克雷伯杆菌临床分离株的耐药性及β内酰胺酶的流行性分析
- Author:
Xiao-Li CAO
1
;
Xue-Jing XU
;
Zhi-Feng ZHANG
;
Han SHEN
;
Ming-Zhe NING
;
Wan-Qing ZHOU
;
Kui ZHANG
;
Bo ZHENG
;
Yuan LV
Author Information
1. 南京大学 医学院 附属鼓楼医院 检验科
- Keywords:
AmpC;
ESBLs;
carabpenease;
cefoxitin;
resistance
- From:
The Chinese Journal of Clinical Pharmacology
2014;(10):919-921
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the resistance of clinically cefoxitin -non -susceptible Klebsiella pneumoniae isolates and the prevalence ofβ-lactamase.Methods WHONET 5.6 was used to analyze the resist-ance of 62 strains with non -susceptibility to cefoxitin recovered.Chi -square test was adopted to compare the resistance rates of 62 isolates with the 239 Klebsiella pneumoniae isolates susceptible to cefoxitin collected during the same period.Meanwhile, the 62 clinical isolates were further taken for preliminary screening for ESBLs and carbapenmase by disk -diffusion tests and modified Hodge tests, respectively.Further analysis for detecting the resistant genes encoding AmpC and carbapenemase was carried out by PCR and DNA sequencing.Results On the whole, cefoxin -non - susceptible Klebsiella pneumoniae isolates displayed higher resistances towards all the antimicrobial agents tested ( P <0.05), except for ampicilin.Preliminary screening test showed that 55 strains (88.7%) were ESBLs producers and 39 strains (62.9%) were carbapenemase producers.PCR and DNA sequencing showed that 8 (12.9%) produced AmpC and 39 (62.9%) strains produced KPC carbapenemase, respectively.Conclusion Most of the Klebsiella pneu-moniae isolates non - susceptibilitible to cefoxitin produce KPC carbapenemase and ESBLs, with AmpC being prevalent, posing a great challenge to clinical therapy and infection control.
