Simultaneous determination of four kinds of domestic and imported anti-epilepsy drugs in human plasma by UPLC-MS/MS
10.13699/j.cnki.1001-6821.2014.09.017
- VernacularTitle:同时测定人血浆中4种国产与进口抗癫痫药浓度的超高效液相色谱-质谱联用法的建立
- Author:
Wei WANG
1
;
Yong-Suo LIU
;
Shi QIAO
;
Qing-Yan LI
Author Information
1. 民用航空医学中心 民航总医院 航空医学研究所
- Keywords:
phenobarbital;
phenytoin sodium;
topiramate;
levertirace-tam;
UPLC-MS/MS
- From:
The Chinese Journal of Clinical Pharmacology
2014;(9):807-809,815
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the method for concentration determi-nation of phenobarbital , phenytoin sodium , topiramate and levertiracetam in human plasma.Methods UPLC -MS/MS was adopted to analyze plasma with protein precipitated by methanol.Sulfamethlazole (SMZ) and pioglitazone hydrochloride were as internal standard.Plasma samples were separated on ACQUITY UPLC HSS PFP column with aqueous solution (0.1%formic acid-5 mmol· L-1 ammonium acetate buffer )-methanol as mobile phase , and at a flow rate of 0.2 mL· min-1.Multiple reaction monitoring ( MRM) mode was performed combined with the ion switching technology for quantification in three sections.Results The liner calibra-tion curve of phenobarbital , phenytoin sodium , topiramate and levertirace-tam were obtained in the concentration range of 1.15 -230.00 ( r =0.997 3 ) , 0.10-20.20 ( r=0.998 5 ) , 0.02-2.12 ( r =0.996 5 ) and 0.10-20.40μg· mL-1(r=0.996 3), respectively.The lowest detection limit were 230.0, 20.20, 5.30,20.40 ng· mL-1 , respectively.The RSD of inter-day and intra -day were less than 15%.The relative recovery was more than 75%.Conclusion The method is accurate , sensitive and suitable for blood concentration monitoring and pharmacokinetic study of phenobarbital , phenytoin sodium , topiramate and levertiracetam.
