Influence of 4 anti-tumor traditional Chinese medicine injections on activities of cytochrome P4503A4 in rat liver microsomes

VernacularTitle:4种抗肿瘤中药注射液对大鼠细胞色素P4503A4亚型酶活性的影响
Author: Xu-Ping YANG ¹ ; Bi-Min FENG ; Yi-Lan HUANG ; Xiao-Yan ZHONG
Author Information

1. 泸州医学院附属中医医院药剂科
Keywords: anti -tumor traditional Chinese medicine injections; liver microsomes; cytochrome P4503 A4; drug-drug interaction
From: The Chinese Journal of Clinical Pharmacology 2014;(9):800-802
CountryChina
Language:Chinese
Abstract: Objective To investigate the influence of elemene , kang-laite, brucea jananica oil and Aidi injection on CYP 3A4 of liver micro-somes in vitro with testosterone as probe.Methods HPLC method was used to detect the production of 6β-hydroxytestosterone (6-OHTS) to study the effects of 4 anti-tumor traditional Chinese medicine injections on the activities of CYP 3 A4 enzymes in the microsomal incubation sys-tem.Results Compared with blank control group , elemene have no sig-nificant effects on CYP3A4; kanglaite, Aidi have a induction on CYP3A4;brucea jananica oil have a inhibition on CYP3A4, IC50 of bru-cea jananica oil on CYP3A4 was 0.78%.Conclusion Potential drug-drug interaction should be vigilant when kanglaite , aidi and brucea ja-nanica oil co -administered with the substrates which mainly undergo CYP3A4-mediated metabolism.