Study on valproic acid induced tumor cell cycle arrest
10.13699/j.cnki.1001-6821.2014.09.012
- VernacularTitle:丙戊酸钠诱导白血病细胞周期阻滞的机制研究
- Author:
Li-Hong WANG
1
;
Zhi-Hua ZHANG
;
Song-Yan ZHU
;
Lei ZHAO
;
Chang-Lai HAO
Author Information
1. 承德医学院 附属医院
- Keywords:
valproic acid;
cell cycle;
cyclin;
cyclin-dependent kinase
- From:
The Chinese Journal of Clinical Pharmacology
2014;(9):791-793
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism of valproic acid ( VPA) inducing tumor cell cycle arrest , by determining the expres-sion of cyclins and cyclin -dependent kinases ( CDKs).Methods Nude mice model with xenograft Kasumi -1 tumor was established by subcuta-neous inoculation of Kasumi -1 cells, and devided two groups: control group and VPA treatment group ( intraperitoneal injection of VPA 500 mg· kg-1 for 2 weeks).The expression levels of cyclinD1, cyclinE1, CDK4, CDK6, CDK2 mRNA were determined by semi -quantitative RT-PCR.The expression level of cyclinD 1 protein was determined by Western blot.Results Compared with the control group , VPA signifi-cantly reducted the expression of cyclinD 1, cyclinE1, CDK4, CDK6, CDK2 mRNA( P<0.05 );and reduced the expression of cyclinD 1 protein ( P<0.01 ).Conclusion The mechanism that VPA inductes cell cycle arrest is possibly due to downregulation of cyclin D 1, CDK4/6, cyclin E and CDK2 expression.
